Plasmid_Backbone

Part:BBa_K814015:Design

Designed by: George Chao, Misha Patel, Hannah Aho, Molly Swanson, Dana Morrone   Group: iGEM12_Minnesota   (2012-10-03)


G418 selection yeast shuttle vector designed for BioBrick


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5682
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5688
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5682
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5682
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5682
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5697
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 5355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 4705


Design Notes

A number of mutations were directed so as to maintin the uniqueness of BioBrick cut sites.


Source

This plasmid was assembled though Gibson assembly. Gibson assembly pieces were amplified from preexisting plasmid templates.

References